Custom Peptide Synthesis

The information contained in this web page is provided “as is” and is for general information purposes only. We do not provide this service on regular basis.

CanadaBioLabs custom peptide synthesis and peptide libraries are offered from 2 to 120 amino acids to include modifications of standard peptide structures, including phosphorylation, methylation, labelling with tags or dyes, cyclization, conjugation with PEG, and incorporation of D-amino acids. CanadaBioLabs custom peptide synthesis can provide custom peptides as trifluoroacetic acid (TFA) salts, acetate salts or hydrochloride salts. CanadaBioLabs custom peptide synthesis has been used in proteomics, antibody products, lead discovery in drug development, etc. CanadaBioLabs peptide chemists help you design and synthesize reliable and validated custom peptides.

CanadaBioLabs custom peptide synthesis services provide custom peptides in several purity levels. Typical purity levels are:

• Immunological Grade (suitable for forming polyclonal antibodies)
• 80% or greater (tissue culture; ligand for affinity purification; non-quantitative antibody blocking experiments)
• 90% or greater (in vivo studies; bioassays; markers for electrophoresis; monoclonal antibodies)
• 95% or greater (ELISA; RIA; enzyme substrate)
• 98%(NMR; chromatography standards)

In selecting a custom peptide synthesis company, factors to consider in addition to cost are accuracy, success rate and speed of delivery.

Our peptides are available in all scales, from milligram research scale to multi-kilogram production scale. CanadaBioLabs provide single custom peptides, multiple peptides and custom peptide libraries in a timely manner at competitive prices.

CanadaBioLabs’s experienced peptide chemists quickly and efficiently prepare most peptide sequences. Custom peptide synthesis is Canada Peptides’s specialty.

Our chemists have experience and expertise in preparing long peptides in high purity. For example, CanadaBioLabs has synthesized and delivered 78 to 81 amino acid custom peptides at 98% purity.

During each custom peptide synthesis, CanadaBioLabs chemists utilize UV monitoring and in-process tests, such as the Kaiser test, to assure each synthesis step is complete before proceeding with the synthesis. If necessary, double coupling protocols are used to assure complete coupling. In addition, heating may be utilized to accelerate slow or difficult reactions. By assuring complete coupling of each amino acid, Canada Peptides chemists maximize overall yield, minimize impurities and reduce the amount of purification required for each custom peptide.

In custom peptides containing hydrophobic sequences where hydrogen bonding of the peptide backbone can result in slow or incomplete reactions, Canada Peptides chemists may utilize hydrogen-bond disrupting elements such as pseudoprolines or Dmb-protected amino acids. In some cases, Boc chemistry with in situ neutralization affords higher yields than Fmoc chemistry.

CanadaBioLabs chemists take steps to minimize racemization of the activated amino acid in coupling which leads to undesired diasteromeic peptides. Racemization during activation is minimized by activating the amino acid residue at reduced temperature and adding HOBt which suppresses the rate of racemization. Cysteine and histidine are especially prone to racemization with uronium activating reagents. When racemization of these residues is especially troublesome, CanadaBioLabs chemists may use carbodiimide/HOBt activation.

All custom peptide products are provided with a complete quality control package, including HPLC and Mass Spectral Analysis, to confirm the purity and identity of the peptide. From 2 amino acids to 85 amino acids, Canada Peptides can prepare peptides in the scale and purity required. Canada Peptides comply with the most stringent quality control specifications for a reasonable price.

CanadaBioLabs Custom Peptide Purity Levels

Immunological Grade: suitable for forming polyclonal antibodies

80% or Greater: tissue culture; ligand for affinity purification; non-quantitative antibody blocking experiments

90% or Greater: in vivo studies; bioassays; markers for electrophoresis; monoclonal antibodies

95% or Greater: ELISA; RIA; enzyme substrate

98%: NMR; chromatography standards

Peptide Synthesis Services

Peptide synthesis services that CanadaBioLabs provides include incorporation of non-standard amino acids, modifications at the N-terminal, C-terminal or side chains, and the formation of cyclic structures. Non-standard amino acids can usually be incorporated into a peptide synthesis with little difficulty. Highly expensive building block, such as isotope-labelled amino acids, are incorporated most efficiently and cost effectively near the N-terminal.

N-Terminal Peptide Modifications

N-terminal modifications are made for a variety of purposes. Some modifications improve the stability of the peptide to proteolytic enzymes or improve the absorption and bioavailability of the peptide. Other N-terminal modifications introduce a dye or tag that allows areas where the peptide accumulates to be visualized. Some modifications are immunoadjuncts to enhance antibody production or linkers to allow conjugation of the peptide to other biomolecules.

List of All Modifications

C-Terminal Peptide Modifications

C-terminal modifications, except for amidation, are less common. They are incorporated either through the utilization of special resins or solution phase reactions after cleavage from the resin. C-terminal modifications can improve the stability of the peptide or alter its interaction with receptors. C-terminal modifications are also used to produce substrates used in enzymatic studies.

List of All Modifications

Internal Modifications

Internal modifications are incorporated into synthetic peptides by using unusual or specially derivatized amino acids. Some internal modifications, such as D-amino acids, beta-amino acids, and N-methyl amino acids, improve peptide stability to enzymes. Unusual amino acid modifications are often used to optimize peptide binding and selectivity in binding to target molecules or insertion into membranes.. Some internal modifications correspond to post translational modifications in proteins. Some examples are phosphorylation, methylation of lysine or arginine side chains, and the acetylation of lysine side chains. These are often involved in the regulation of biological processes. Amino acids containing stable heavy isotopes are used to label peptides in mass spectra studies.

List of All Modifications

Cyclization

Cyclization of peptides enhances conformational stability, can mimic protein secondary structures and enhance protease stability. Cyclization can be achieved in a number of different ways – by disulfide bond formation betCanadaBioLabs en cysteine residues, by head-to-tail amide bond formation, by ring-closing metathesis (stapling), or by amide bond formation betCanadaBioLabs en side chains or a side chain and the N- or C-terminal of the peptide.

Additional Services

CanadaBioLabs can provide the following services:

• Amino Acid Analysis
• Aliquoting
• TFA Removal

Large Scale Custom Peptide Production

CanadaBioLabs can perform custom peptide synthesis in larger scales, from gram to multi-kilogram quantities. CanadaBioLabs complies with the most stringent quality control specifications at a competitive price. From our extensive experience in custom peptide synthesis, to our Canada Peptides ll-trained technical team and highest production standards, CanadaBioLabs hope to be your strong and reliable partner in the life sciences industry.

Custom Peptide Libraries

Custom peptide libraries from CanadaBioLabs are provided in 96-Canada Peptides ll plates with approximately 2 μmol of lyophilized peptide in each CanadaBioLabs ll. CanadaBioLabs can synthesize peptide libraries for drug discovery, high-throughput screening, and SAR studies, including alanine scans and truncation sets. CanadaBioLabs utilizes optimized protocols and chemistries to ensure consistent purity, quantity, and quality library-to-library and within each peptide library.

CanadaBioLabs can incorporate most standard peptide modifications within custom peptide libraries. Standard modifications that can be incorporated are unusual amino acids, D-amino acids, C-terminal amidation, N-terminal modification, and biotin, dye or fluorescence labeling on the N-terminal or lysine side chains.

Custom libraries are typically completed in 2-3 CanadaBioLabs eks. Typical delivery consists of lyophilized peptides in 96-CanadaBioLabs ll titer plates, peptide location table, and MS and HPLC data.